Satellite Systems
- ACTRI AX R
- CMM-East X1
- Pacific Hall AX R
- Pacific Hall W1 SoRa
Nikon’s newest point scanning confocal offering an industry leading 25mm field of view, hybrid spectral and filter-based detection, up to 8K resolution galvo scanning and 2K high speed resonant scanning with 405/488/561/640 excitation. Also offers incubation, DIC imaging, fast Z-section capabilities, and automatic water dispenser for high-throughput, high-resolution experiments.
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Specifications |
Microscope body |
Eclipse Ti2-E |
Stage |
Automated |
Piezo Drive |
Queensgate 600µm |
Perfect focus system (PFS) |
Yes |
Incubator |
Tokai Hit Stage top (25 – 50 ⁰C, CO 2, and humidity) |
Objectives |
Correction, Magnification, Numerical Aperture, Working Distance |
Plan Apo ʎ 4x N.A. 0.2 air, WD 20000 µm Plan Apo ʎ 10x NA 0.45 air, WD 4000 µm Plan Apo ʎ 20x NA 0.75 air, WD1000 µm Plan Apo ʎ 40x NA 1.15 water, WD 590-610 µm Plan Apo ʎ 60x NA 1.40 oil, WD 130µm |
|
Software |
NIS Elements software (version 5.42.02: High content analysis package) |
Lasers |
405 nm, 488 nm, 561 nm, and 640 nm (LUA-S4) |
Scanning modalities |
Galvano- up to 8k frame size Resonant- up to 4k frame size |
Detectors |
DUX-VB 418-475 nm multi-alkali PMT 400-750nm GaAsP variable range (typically 499-543 nm, 571-625 nm) 655-850 nm GaAsP IRIS 15 15 Megapixel Camera 4.25 µm x 4.25 µm Pixel Area Large 25 mm Diagonal Field of View |
Filters |
DAPI filter- 447/57 CFP filter- 482/35 Cy5 filter -640 (Short Pass. For reflecting 640 emission to fixed detector.) Cy7 filter- 730 (Short Pass. For reflecting 730 emission to fixed detector.) |
Dichroic mirrors |
405/488/561/640 |
Beam splitter |
80/20 |
Spectrum scanning |
400 – 750 nm bandwidth of 5, 10, 20, 40nm 9-70 channels 32PMT detectors |
ACTRI L2-W420