CMM-East X1
Summary
Spinning disk confocal offering fastest possible live-cell imaging with 405/488/561/640 excitation. Paired with an Ixon Life 888 EMCCD camera for high sensitivity imaging of dim or challenging samples. Also offers fast Z-sectioning capabilities (piezo), thermal stage control from 0C – 100 C and DIC imaging.
Imaging modalities
- Yokogawa CSU-X1 spinning disk
- Bright field and DIC
Applications
- Prolonged fluorescence live cell imaging. There are 4 different laser lines available for the spinning disk confocal. A fluorescence mode can also be combined with brightfield or DIC.
- (Fast) live cell imaging. Spinning disk illumination is very mild for your sample, which is beneficial when imaging fluorescent proteins. A thermal stage controlled Peltier (0 – 100 ⁰C).
- FRAP and stimulation experiments. You can, activate or convert with the 405 nm laser simultaneous. Or you can bleach, activate or stimulate with the 405 nm, 488 nm, 561 nm or 640 nm laser and image sequentially. The stimulation function is integrated in the NIS elements software. Cells can be immediately analyzed after or even during the acquisition with the time measurement tool.
Technical description
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Specifications |
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Microscope body |
Eclipse Ti2-E |
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Stage |
Automated |
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Piezo Drive |
Queensgate 600µm |
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Perfect focus system (PFS) |
Yes |
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Incubator |
Temperature controlled Peltier |
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Objectives |
Correction, Magnification, Numerical Aperture, Working Distance |
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Plan ApoD ʎ 10x D NA-.45 air, WD 4000 µm Plan Fluor 40x NA-1.30 oil, WD 200 µm Plan Apo ʎD 40X, N.A.0.95 air, WD 170-250 µm Plan Apo ʎD 60x NA-1.42 oil, WD 150 µm Plan Apo ʎD 100x NA-1.45 oil, WD 130 µm |
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Software |
NIS elements software (version 5.42.02: High content analysis package) |
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Lasers |
405 nm, 488 nm, 561 nm, 640 nm (LUNF-XL box) |
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Yokogawa spinning disk |
CSU-X1-50 µm pinholes |
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FOV |
10x7mm |
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Detectors |
iXon Life 888 Sensor format 1024 x 1024 Sensor diagonal 18.8 mm QE Options BV (Life) Pixel Size 13 µm Frame Rate 26 fps (670 fps with 128 x 128 Crop Mode) Read Noise < 1 e- with EM Gain Pixel well depth 80,000 e- |
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Emission filters |
DAPI-455/50 GFP-525/36 TRITC-605/52 CY5-705/72 Quad (DAPI/FITC/TRITC/CY5) Polarizer |
Location
CMM East