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CMM-East X1

Summary

Spinning disk confocal offering fastest possible live-cell imaging with 405/488/561/640 excitation. Paired with an Ixon Life 888 EMCCD camera for high sensitivity imaging of dim or challenging samples. Also offers fast Z-sectioning capabilities (piezo), thermal stage control from 0C – 100 C and DIC imaging.

Imaging modalities

  • Yokogawa CSU-X1 spinning disk
  • Bright field and DIC

Applications

  • Prolonged fluorescence live cell imaging. There are 4 different laser lines available for the spinning disk confocal. A fluorescence mode can also be combined with brightfield or DIC.
  • (Fast) live cell imaging. Spinning disk illumination is very mild for your sample, which is beneficial when imaging fluorescent proteins. A thermal stage controlled Peltier (0 – 100 ⁰C).
  • FRAP and stimulation experiments. You can, activate or convert with the 405 nm laser simultaneous. Or you can bleach, activate or stimulate with the 405 nm, 488 nm, 561 nm or 640 nm laser and image sequentially. The stimulation function is integrated in the NIS elements software. Cells can be immediately analyzed after or even during the acquisition with the time measurement tool.

Technical description

 

Specifications

Microscope body

 Eclipse Ti2-E

Stage

 Automated

Piezo Drive

 Queensgate 600µm

Perfect focus system (PFS)

 Yes

Incubator

 Temperature controlled Peltier

Objectives

Correction, Magnification, Numerical Aperture, Working Distance

 Plan ApoD ʎ 10x D NA-.45 air, WD 4000 µm

 Plan Fluor 40x NA-1.30 oil, WD 200 µm

 Plan Apo ʎD 40X, N.A.0.95 air, WD 170-250 µm

 Plan Apo ʎD 60x NA-1.42 oil, WD 150 µm

 Plan Apo ʎD 100x NA-1.45 oil, WD 130 µm

Software

 NIS elements software (version 5.42.02: High content analysis package)

Lasers

 405 nm, 488 nm, 561 nm,  640 nm (LUNF-XL box)

Yokogawa spinning disk

 CSU-X1-50 µm pinholes

FOV

 10x7mm

Detectors

 iXon Life 888

 Sensor format            1024 x 1024   

 Sensor diagonal         18.8 mm

 QE Options                  BV (Life)

 Pixel Size                     13 µm 

 Frame Rate                  26 fps (670 fps with 128 x 128 Crop Mode) 

 Read Noise                   < 1 e- with EM Gain   

 Pixel well depth           80,000 e-

Emission filters

 DAPI-455/50

 GFP-525/36

 TRITC-605/52

 CY5-705/72

 Quad (DAPI/FITC/TRITC/CY5)

 Polarizer

 

Location

CMM East