Large field of view Widefield microscope

Summary

This is a widefield microscope with a large field of view and is equipped for 3 imaging modalities: brightfield, Differential Interference Contrast (DIC), and widefield fluorescence. The biggest advantage of this system is the large field of viiew; every imaging mode benefits from this. This large field of view shows even multiple cells with subcellular details at 100x magnification! Complete with auotmation in combination with perfect focus system making this an ideal system to acquire entire slides and 96 well plates in an unattended fashion. This could even be done as a time lapse with duration of hours or even overnight and longer.

Imaging Modalities

• Brightfield
• DIC imaging
• Widefield fluorescence imaging 

Applications 

• Immunofluorescence. There are 5 different LED colors with matching filters available in the widefield fluorescence imaging mode. 
• Fluorescence live cell imaging. In the widefield fluorescence mode there are 6 different LED excitation colors available which are matched with appropriate filters. The stage top incubation chamber regulates temperature, CO₂ and humidity.
• Organoid imaging. The large field of view is ideal to acquire an entire organoid in one frame. In addition, long working distance objectives are available to image through the entire organoid.
• DIC and brightfield. DIC and brightfield can be used as standalone image modes or can be combined with fluorescence or color imaging. The stage top incubation chamber is fully DIC compatible. 

Technical Description

Location

CMM East 3071