Pacific Hall AX R

Summary

Nikon’s newest point scanning confocal offering an industry leading 25mm field of view, hybrid spectral and filter-based detection, up to 8K resolution galvo scanning and 2K high speed resonant scanning with 405/488/561/640 excitation. Also offers incubation, DIC imaging, and Z-section capabilities for high-throughput, high-resolution experiments.

Imaging modalities

• Laser scanning confocal – Galvano and Resonant scanner
• Spectral imaging
• Bright field and DIC

Applications

• Immunofluorescence samples. Imaging entire slides is easy and straightforward using tiling and stitching functions in the NIS-Elements software. You can control the orientation of the Galvo scanner and thereby control the orientation of your sample within the image.
• Live cell imaging. Tokai Hit stage top incubation chamber regulates temperature (25 – 50 ⁰C), CO₂ and humidity. The resonant scanner is ideal to image fast moving processes with bright fluorophores.
• FRAP and stimulation experiments. You can image with the resonant scanner and use the Galvo scanner to simultaneously stimulate, activate or convert with the 405 nm laser simultaneous. Or you can bleach, activate or stimulate with the 405 nm, 488 nm, 561 nm or 640 nm laser and image sequentially using only the Galvo scanner. The stimulation function is integrated in the NIS elements software. Cells can be immediately analyzed after or even during the acquisition with the time measurement tool.
• Spectral imaging. The spectral range is 400 – 750 nm with a bandwidth of 5, 10, 20, 40nm with a range of 9-70 channels depending on bandwidth.
• Image ‘exotic’ and large Stokes shift fluorophores. The tunable emission width in combination with 405, 488, 561 or 640 nm lasers provide many acquisition options for a wide variety of fluorophores.

Technical description

Location

Pacific Hall 3225A